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. 2018 Aug 15;8:279. doi: 10.3389/fcimb.2018.00279

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Copyright © 2018 Wang, Wang, Wang, Meng, Song, Huo, Zhang, Chang, Li, Zheng, Liu, Zhang, Zhang and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of non-structural proteins 3D and 3C on G0/G1-phase arrest. (A) The cell cycle distribution in 293T cells was analyzed at 36 h after transfection with 0, 0.5, 1, 2, or 4 μg plasmid as indicated. (B) The histograms indicating the cell cycle distribution were analyzed by the ModFit LT program. The results indicate the mean ± SD of three independent experiments. (C) The cell cycle distribution and fluorescence density in 293T cells were analyzed at 36 h after transfection with 4 μg PEGFP (Vec), 3C-PEGFP (3C) and 3D-PEGFP (3D) plasmid. (D) The histograms displaying the cell cycle distribution were analyzed by the ModFit LT program. Top panel: Cells with green fluorescence indicated successful transfection. Bottom panel: Cells without green fluorescence indicated non-successful transfection. The results indicate the mean ± SD of three independent experiments. ^***P < 0.001.