Table 1.
Extraction recovery and matrix factor for tadalafil from human plasma.
| Quality control level (ng/mL) | Mean area response (n = 6) |
Recovery (%) |
Matrix factor |
|||||
|---|---|---|---|---|---|---|---|---|
| A (post-extraction spiking) | B (pre-extraction spiking) | C (neat samples in mobile phase) | Analyte (B/A) | IS | Analyte (A/C) | IS | IS-normalized (Analyte/IS) | |
| 400 | 5,452,803 | 5,447,895 | 5,558,413 | 99.91 | 99.84 | 0.981 | 0.976 | 1.005 |
| 200 | 2,762,572 | 2,758,151 | 2,833,407 | 99.84 | 100.05 | 0.975 | 0.981 | 0.994 |
| 30.0 | 418,560 | 421,113 | 436,910 | 100.61 | 99.63 | 0.958 | 0.972 | 0.986 |
| 1.50 | 20,735 | 20,689 | 21,895 | 99.78 | 101.52 | 0.947 | 0.928 | 1.020 |
| 0.50 | 6816 | 6744 | 7172 | 98.95 | 99.58 | 0.953 | 0.950 | 1.003 |
IS: internal standard.