Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used for site-directed mutagenesis.
| Mutagenesis | Direction of primer | Primer sequence (5′–3′) | Annealing temp (°C) |
|---|---|---|---|
| blaKLUC-5D240G | Forward | TAAAACCGGCAG CGGTGGTTACGGCACC | 63 |
| Reverse | CCACCGCTGCCGGTTTT ATCGCCCACCA | ||
| blaKLUC-5S18G | Forward | TTCCGCTG CTGGCAGGCAGCGTATCG | 63 |
| Reverse | CTGCCAGCAGCGGAA AGACCGTCGCG | ||
| blaKLUC-3G240D | Forward | AACCGGCA GCGGTGATTACGGCACCA | 63 |
| Reverse | TCACCGCTGCCGGTTTT ATCGCCCACC |
