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Recombinant fragment of human SP-D (rfhSP-D) expression and purification. Escherichia coli BL21 (λDE3) pLysS containing pUK-D1 was induced with IPTG. The expressed bacterial cells show rfhSP-D over-expression at ~20 kDa as compared to un-induced cells. The insoluble inclusion bodies were refolded and affinity purified on maltose-agarose. The peak fractions shown here appeared homogenous on a 12% SDS-PAGE.
