Figure 4.

Recombinant fragment of human SP-D (rfhSP-D) supresses invasiveness in pancreatic cancer cell lines. (A) The cell invasion was analyzed by incubating 35,000 cells with and without rfhSP-D (20 µg/ml) in the BioCoat™ Matrigel™ Invasion Chambers at 37°C for 22 h. The invasive cells were fixed and stained before mounting the membrane on the slide for cell counting. The images show the difference between treated and untreated. (B) Treatment with rfhSP-D significantly reduced the cell invasion as for Panc-1 (~50%) and MiaPaCa-2 (~65%). However, as anticipated, no invasion was detected in Capan-2 cell line, neither in treated nor untreated samples. Significant values were considered based on ***p < 0.001 and ****p < 0.0001 between treated and untreated samples.