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Calcification. (A) Tile scan of a healthy ovine aortic valve stained with osteocalcin (OCN). (B) Section of the ovine pulmonary wall in which the onset of calcification was detected, stained against OCN. (C) Russell Movat's pentachrome staining of the same section of pulmonary wall revealed abundant presence of proteoglycans (in blue-green), colocalizing with OCN expression. Scale bars, 1 mm (A) and 100 μm (B, C).
