Table 1.
Antibodies used for immunohistochemistry.
| Antibody | Host | Clone | Distributor | Cat. No. | Antigen retrieval | Dilution |
|---|---|---|---|---|---|---|
| ECM | ||||||
| Collagen type 1 | Mouse | Col-1, IgG1 | Sigma | C2456 | Citrate | 200 |
| Collagen type 3 | Rabbit | Polyclonal | Abcam | Ab7778 | Pepsin | 250 |
| Periostin | Rabbit | Polyclonal | n.a. | n.a. | Citrate | 500 |
| Versican | Rabbit | Polyclonal | Abcam | Ab6994 | Proteinase K | 200 |
| Biglycan | Rabbit | Polyclonal | Biorbyt | orb100396 | Pepsin | 150 |
| Decorin | Rabbit | Polyclonal | Biorbyt | orb10520 | Tris/EDTA | 150 |
| MMP-1 | Rabbit | EP1247Y, IgG | Abcam | ab52631 | Citrate | 200 |
| MMP-2 | Mouse | 6E3F8, IgG2b | Abcam | ab86607 | Proteinase K | 400 |
| MMP-9 | Rabbit | Polyclonal | Abcam | ab38898 | Citrate | 200 |
| MMP-13 | Rabbit | Polyclonal | Abcam | ab39012 | Citrate | 200 |
| TGF-β1 | Rabbit | Polyclonal | Abcam | ab9758 | Citrate | 500 |
| TGF-β3 | Rabbit | Polyclonal | Novus Biologicals | NB600-1531 | Citrate | 400 |
| ELASTOGENESIS | ||||||
| (Tropo)elastin | Rabbit | Polyclonal | Abcam | ab21610 | Pepsin | 400 |
| Fibronectin | Rabbit | Polyclonal | Sigma | F3648 | Pepsin | 300 |
| Fibrillin-1 | Rabbit | Polyclonal | Sigma | HPA017759 | Pepsin | 100 |
| Fibrillin-2 | Rabbit | Polyclonal | Sigma | HPA012853 | Pepsin | 100 |
| Lysil oxidase (LOX) | Rabbit | Polyclonal | Novus Biologicals | NB100-2530 | Tris/EDTA | 200 |
| Fibulin-4 | Rabbit | Polyclonal | Abonline | abin1701235 | Pepsin | 200 |
| Fibulin-5 | Mouse | 3F10A5, IgG1 | Abonline | abin1107239 | Proteinase K | 100 |
| EMILIN-1 | Rabbit | Polyclonal | Biorbyt | orb183385 | Pepsin | 200 |
| VICs | ||||||
| α-SMA | Rabbit | Polyclonal | Abcam | ab5694 | Pepsin/citrate | 600 |
| Vimentin | Rabbit | D21H3, IgG | Cell Signalling | 5741 | Citrate | 300 |
| SM22 | Rabbit | Polyclonal | Abcam | ab14106 | Citrate | 200 |
| Calponin | Rabbit | Polyclonal | Abcam | ab46794 | Pepsin | 150 |
| Cadherin-11 | Rabbit | Polyclonal | Thermo Scientific | 71-7600 | Citrate | 100 |
| SMemB | Mouse | 3H2, IgG2b | Abcam | ab684 | Tris/EDTA | 200 |
| VECs | ||||||
| CD31 (PECAM-1) | Mouse | CO.3E-1D4, IgG2a | Novus Biologicals | NB100-65900 | Pepsin | 100 |
| CD34 | Rabbit | EP373Y, IgG | Abcam | ab81289 | Tris/EDTA | 200 |
| VE-Cadherin | Rabbit | Polyclonal | Abcam | ab33168 | Citrate | 200 |
| von Willebrand factor | Rabbit | Polyclonal | Abcam | ab6994 | Citrate | 1200 |
| PROLIFERATION/APOPTOSIS | ||||||
| Ki-67 | Rabbit | Polyclonal | Thermo Scientific | rb1510-PO | Citrate | 200 |
| Cleaved Caspase-3 | Rabbit | Polyclonal | Cell Signalling | 9661 | Citrate | 200 |
| CALCIFICATION | ||||||
| Osteocalcin | Rabbit | Polyclonal | Abcam | ab93876 | Tris/EDTA | 200 |
| INFLAMMATION | ||||||
| CD45 | Mouse | 1.11.32, IgG1 | AbD-serotec | MCA2220GA | None | 1000 |
| CD3 | Rabbit | Polyclonal | DAKO | A0452 | Tris/EDTA | 200 |
| CD64 | Mouse | 3D3, IgG1 | Abcam | ab140779 | Citrate | 200 |
| CSF-1R (CD115) | Rabbit | Polyclonal | Abonline | abin498117 | Citrate | 200 |
| EMR-1 | Rabbit | Polyclonal | Gene Tex | GTX101895 | Tris/EDTA | 500 |
| iNOS | Rabbit | Polyclonal | Abcam | ab3523 | Citrate | 400 |
| CCR7 (CD197) | Rabbit | Polyclonal | Abcam | ab32527 | Citrate | 1200 |
| TNF-a | Rabbit | Polyclonal | Acris | AP20373PU-n | Citrate | 300 |
| CD163 | Mouse | EdHu-1, IgG1 | AbD-serotec | mca1853 | Citrate | 250 |
| CD200R | Rabbit | Polyclonal | Abonline | Abin737586 | Citrate | 200 |
| Arginase-1 | Rabbit | Polyclonal | Bioss | bs-8585R-Biotin | Citrate | 500 |
| IL-10 | Rabbit | Polyclonal | Biorbyt | orb221323 | Citrate | 200 |
| CXCR4 (CD184) | Mouse | 44716, IgG2b | RnD systems | MAB172 | Citrate | 500 |
| CD44 | Rabbit | Polyclonal | Abcam | ab24504 | Citrate | 250 |
MMP, matrix metalloproteinase; TGF-β, transforming growth factor-β; EMILIN-1, elastin microfibril interfacer 1; α-SMA, α-smooth muscle actin; CSF-1R, colony stimulating factor-1 receptor; EMR-1, EGF-like module-containing mucin-like hormone receptor-like 1; iNOS, inducible nitric oxide synthase; TNF-α, tumor necrosis factor-α; IL-10, interleukin-10: VE-cadherin, vascular endothelial cadherin