Figure 7.

NSE spectroscopy of α-catenin dimer. (A), (B) and (C) show intermediate correlation function I(Q,t)/I(Q,0) as a function of Fourier time at different Q values. The lines are single exponential fits to the initial slope of I(Q,t)/I(Q,0). Note that at Q between 0.0509–0.1379 Å⁻¹I(Q,t)/I(Q,0), shows non-single exponential behavior. (D) The effective diffusion constants D_eff(Q) (black squares) obtained from the initial slopes of the spectra in (A), (B) and (C) are shown. The solid red line is the theoretical D_eff(Q) using the coordinates of α-catenin dimer shown in Fig. 4C, assuming the α -catenin dimer is a rigid body. The dashed red line is the theoretical D_eff(Q), assuming amino acid residues 836–906 in each protomer of the dimer are moving. (E) The dynamics of α-Catenin dimer colored by normal mode analysis, with blue representing low mobility and red high mobility, is shown.