Figure 3.

Analysis of IHC ribbon synapses in unexposed WT, Chrna9 KO, and Chrna9L9′T KI mice. Quantitative data obtained from unexposed WT, Chrna9 KO, and Chrna9L9′T KI mice at P28. For each IHC, we analyzed the number of CtBP2 puncta, postsynaptic GluA2 receptor patches, and putative ribbon synapses, defined as juxtaposed CtBP2 and GluA2 positive puncta. Horizontal lines inside the boxplots represent the median and whiskers correspond to percentiles 10–90. Comparisons were made by a Kruskal–Wallis nonparametric ANOVA followed by a Dunn's post-test. Dark gray asterisks represent the statistical significance between Chrna9 KO and WT mice, and light gray asterisks between Chrna9L9′T KI and WT mice (*p < 0.05; **p < 0.01; ***p < 0.001).