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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Connect Tissue Res. 2017 May 23;59(2):157–166. doi: 10.1080/03008207.2017.1321639

Figure 3.

Figure 3.

TMF reduced IRE1α siRNA-induced apoptosis after treatment with 1 μM TM for 4 h in chondrocytes. The chondrocytes were incubated for 48 h. Chondrocytes in the negative control (NC) group was incubated without adding any medicines. Group (TM) was the chondrocytes incubated with 1 μM TM for 4 h. Group (IRE1 siRNA) was the chondrocytes transfected with IRE1α siRNA. Group (TM+scrambled siRNA) was the chondrocytes transfected with scrambled siRNA incubated with 1 μM TM for 4 h. Group (TM+ IRE1 siRNA) was the chondrocytes transfected with IRE1α siRNA incubated with 1 μM TM for 4 h. Group (TM+ IRE1 siRNA + TMF) were the groups that the chondrocytes transfected with IRE1α siRNA incubated with 1 μM TM and 5, 20 μg/mL TMF for 4 h, respectively. The summarized data in histogram were indicating the chondrocytes apoptosis ratio, as detected by the DAPI/TUNEL staining. Data were presented by mean ± standard deviation of four replicates. *p < 0.05 compared with control.