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(A) The mRNA expression changes of IRE1α are induced by 5 and 20 μg/mL TMF or in combination of TM for 4 h. (B) The mRNA expression changes of IRE1α, CHOP, XBP1, Bcl-2 and caspase-3 in IRE1α siRNA transfected chondrocytes incubated with 1 μM TM and 5 and 20 μg/mL TMF for 4 h. Changes in mRNA expression of these genes were detected by qRT-PCR. GAPDH was used as internal control. Data were presented by mean ± standard deviation of four replicates. *p < 0.05 compared with control.
