Figure 4.

Differentiation of mesodermal derivatives (myotubes and cartilage) and ability for cell proliferation in teratomas after 2 wk of in vitro culture. A, Myotubes with several nuclei (arrow) found in a teratoma in vitro after hyperthermia of 42°C applied for 15 min (CEM43°C 3.75 min; HE). B, Perinuclear nestin expression in differentiated myotubes (arrow) after hyperthermia of 43°C for 30 min (CEM43°C 210 min) during 7 d. IHC, DAB, counterstained by haematoxylin. C, Cartilage with chondroblasts (arrow), chondrocytes situated in lacunae (dashed arrow) and isogenic groups of chondrocytes (arrowhead) after a two week hyperthermia on 40.5°C starting from the second day (CEM43°C 585 min; HE). D, Expression of PCNA (arrow) in the cartilage (C), in some cells of the neural tissue (N) and epidermis (E) and expression of nestin (arrowhead) in teratoma treated with 42°C for 15 min (CEM43°C 3.75 min). IHC, double immunolabelling, Fast Red, DAB, counterstained by haematoxylin [Colour figure can be viewed at http://wileyonlinelibrary.com]