Table 1.
Expected and observed mutation frequencies after NGS of libraries generated by BAmSeq.
| Sample ID | DNA source | Expected mutant allelic frequency % | Observed mutant allelic frequency % |
|---|---|---|---|
| S1 | gDNA (HCT15 + MCF10A) | 50% | 48% |
| S2 | gDNA (HCT15 + MCF10A) | 25% | 25% |
| S3 | gDNA (HCT15 + MCF10A) | 10% | 13% |
| S4 | gDNA (HCT15 + MCF10A) | 5% | 7% |
| S5 | gDNA (HCT15 + MCF10A) | 3% | 3% |
| T1 | gDNA (U87GM + MCF10A) | 50% | 47% |
| T2 | gDNA (U87GM + MCF10A) | 25% | 21% |
| T3 | gDNA (U87GM + MCF10A) | 15% | 15% |
| T4 | gDNA (U87GM + MCF10A) | 10% | 12% |
| T5 | gDNA (U87GM + MCF10A) | 5% | 6% |
| T11 | Recovered DNA from sample T1 | 50% | 53% |
| T12 | Recovered DNA from sample T2 | 25% | 24% |
Column one: S = SPOP libraries; T = TERT libraries. Column two: source of genomic DNA (gDNA). Column three: expected mutation percentage based on user specified wild type (WT) to mutant ratios. Column four: allelic frequency obtained during sequencing.