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The effects of WNT16 on osteogenic gene expression. PSC were cultured in osteogenic differentiation medium (10 mM β-glycerophosphate and 50 μM ascorbic acid in DMEM + 20% FBS) with or without exogenous WNT16 (80 ng/mL). Gene expression of RUNX2 (0,3,6, and 9 days), ALP (3 days), and OCN (12 days) of differentiation. *p < 0.05, **p < 0.01 versus control.
