Table 2.

Box–Behnken design matrix

Run	T	DO	IPTG	rPDT band intensity
1	20	10	112.5	10934.5
2	40	10	112.5	6874.91
3	20	50	112.5	11531.7
4	40	50	112.5	6147.456
5	20	30	25	10745.06
6	40	30	25	5949.517
7	20	30	200	11619.7
8	40	30	200	6395.547
9	30	10	25	8968.991
10	30	50	25	9389.53
11	30	10	200	9104.195
12	30	50	200	9437.547
13	30	30	112.5	9953.16
14	30	30	112.5	10302.3
15	30	30	112.5	10235