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. 2018 Aug 15;11:282. doi: 10.3389/fnmol.2018.00282

Figure 6.

Figure 6

Targeting CRISPR-dCas9-VP192 to the CGG repeat overcomes transcriptional silencing and selectively enhances transcription of FMR1 in FXS hESCs and neural progenitor cells (NPCs). (A) Relative FMR1 mRNA expression in early (P23–25) passage FXS hESCs transfected with control plasmid or dCas9-VP192 and the indicated gRNA (**p < 0.01, *p < 0.05, Kruskal-Wallis one-way ANOVA with Dunn’s multiple comparison test). (B) Relative FMR1 mRNA expression in late (P53–57) passage FXS hESCs transfected with control plasmid or dCas9-VP192 and the indicated gRNA (**p < 0.01, *p < 0.05, Kruskal-Wallis one-way ANOVA with Dunn’s multiple comparison test). (C) Relative FMR1 mRNA expression in control hESC-derived NPCs at 48 h after transfection with control plasmid or dCas9-VP192 after differentiation with the indicated gRNAs (*p < 0.05, Kruskal-Wallis one-way ANOVA with Dunn’s multiple comparison test). (D) Relative FMR1 mRNA expression in late FXS hESC-derived NPCs transfected with control plasmid or dCas9-VP192 with the indicated gRNAs (*p < 0.05, Kruskal-Wallis one-way ANOVA with Dunn’s multiple comparison test). For all scatter plots, each data point represents an individual well. Data were obtained from two independent experiments. The mean with error bars (SEM) is shown for each condition.