Figure 8.

Targeted reactivation of FMR1 does not significantly enhance FMRP expression in FXS hESCs. (A) Western blots showing triplicate samples of early (P25–28) passage FXS hESCs transfected with control plasmid (eGFP) or with dCas9-VP192 and gRNAs immunoblotted for FMRP (by Femto ECL), Cas9 and tubulin as a loading control. (B) Quantification of western blots from early passage FXS hESCs transfected as indicated. (C) Western blots showing triplicate examples of late (P47–64) passage FXS hESCs transfected as in (A). (D) Quantification of western blots from late passage FXS hESCs transfected as indicated. For all scatter plots, each data point represents an individual well. Data were obtained from two (B) or three (D) independent experiments. The mean with error bars (SEM) is shown for each condition. ns, not significant.