Figure 4.

(a and b) Three-color, polarization-resolved single-molecule images for vertical (a) and horizontal (b) detection SPADs. The colors in the images correspond to the different Di molecules used in the experiments. (c–e) Polarization-resolved images of DiO (c), DiI (d), and DiD (e) molecules, pseudo-color-coded according to the in-plane orientation of the emission dipole, are shown, with green being vertical and magenta horizontal in the images. Molecules oriented at ±45° appear white. Images recorded simultaneously at 488, 561, and 640 nm with a 2-ms pixel dwell time. Scale bars, 5 μm. (f–h) Respective DOLP histograms for each of the three colors are shown. To see this figure in color, go online.