Table 1.

Normalized Effective Excitation Spectra Retrieved by the Unmixing Algorithm, i.e., α_lfI_l Components from Unmixing for the Four-Target Sample, at the Four Different Excitation Wavelengths

	470 nm	514 nm	561 nm	640 nm
Golgi	0.89	0.00	0.00	0.11
Lipid droplets	0.10	0.60	0.21	0.11
Mitochondria	0.10	0.18	0.53	0.20
Nucleus	0.05	0.03	0.12	0.81

The sums of the rows corresponding to each organelle may not exactly equal 1.0 due to rounding. Golgi complex was labeled with eGFP, lipid droplets labeled with BODIPY 500/510, mitochondria labeled with Alexa 532, and nucleus labeled with Alexa 647.