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Determining the alcohol’s bilayer partition coefficients (Kp). The gramicidin-based fluorescence assay was done at different lipid volumes (Vlip). (A) Normalized fluorescence time courses observed with fluorophore-loaded LUVs incubated with gramicidin and 5 mM HFIP and varying lipid volumes are shown. (B) The fluorescence quench doubling rate (Dnom) for HFIP as function of the lipid volume (of Vlip) is shown. To see this figure in color, go online.
