Figure 5.

Transgene-Specific CTL Response Detection after AAV8/hFVIII Administration

The FVIII-specific CTL responses were measured by IFN-γ ELISPOT. The PBMCs from either dog U10 or U11 blood was cultured in the presence of different pools of the FVIII peptides for 48 hr in an ELISPOT plate. The combination of PMA and ionomycin was used as positive controls. IFN-γ-secreting cells (IFN-γ SCs) were detected by ELISPOT assay. The data represented the average and SD (error bar) from triplicates. Control used 10% DMSO in PBS, which was the solvent for the peptides. Data are presented as average ± SE.