Figure 4.

Localization of Col8a1 in mouse retinas. A, The localization of Col8a1 (in red) was studied on P90 retinas derived from wild-type C57BL/6J mice. Laminin β-1 (Lamb1; green) was used as a Bruch’s membrane marker. Col8a1 colocalizes with Lamb1 at Bruch’s membrane. Col8a1 staining also showed a weaker signal in other layers of the retina. B, Magnifications of the outer region of the retina, where the colocalization between Lamb1 and Col8a1 can be appreciated. DAPI (4′,6-diamidino-2-phenylindole) (blue) was used to stain cell nuclei. BM = Bruch’s membrane; GCL = ganglion cell layer; INL = inner nuclear layer; IPL = inner plexiform layer; IS = inner segment; ONL = outer nuclear layer; OPL = outer plexiform layer; OS = outer segment; RPE = retinal pigment epithelium.