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. 2018 Aug 15;9:1192. doi: 10.3389/fpls.2018.01192

FIGURE 2.

FIGURE 2

Expression of OsNRT2s and OsNAR2.1 in transgenic lines. WT and transgenic lines seedlings were grown in the IRRI nutrient solution containing 1 mM NH4+ for 1 month and then treated with 0.5 mM NH4+/NO3- for 2 weeks. Extraction of total RNA from roots of WT and transgenic lines and qRT-PCR under (A) 0.5 mM NH4+, (B) 0.5 mM NO3-. WT, wild type; OE1/2/3 was performed for the three OsNRT2.1 transgenic lines, as below. Error bars: standard error (n = 4 plants). Different letters indicate a significant difference between transgenic and WT lines (P < 0.05, one-way ANOVA).