FIG. 5.

PIV5 V protein suppresses CA-MDA5-induced IFNβ expression. Empty vector, WT, or CA-MDA5 were expressed in HEK293T cells in the presence and absence of PIV5 V protein. Total RNA was collected and analyzed for IFNβ mRNA expression by RT-qPCR. Data are representative of ≥3 replicate experiments and are shown normalized to GAPDH expression. Bars indicate average values of technical replicates (n = 3) with error bars representing standard deviation. Statistical analysis was done using a 2-tailed Student's t-test (*P < 0.05, **P < 0.005).