Fig. 2.

Targeting β1 and αV integrin induces partial inhibition of glioma cell invasion in 3D mouse astrocyte scaffolds. (A) Invasion of U-251 and E-98 cells from spheroids after culture for 2 days in 3D astrocyte scaffolds in the presence of isotypic IgG1 (Iso) and cRADfV control peptide or adhesion-perturbing anti-β1-integrin antibody (4B4) and cRGDfV peptide. (B) Average invasion distance of U-251 and E-98 cells from the spheroid margin and the total number of invaded cells. Data represent 41 and 37 (U-251), or 36 and 43 (E-98) spheroids per control and integrin interference condition, respectively from three independent experiments. Values display the median (black line), 25th and 75th percentiles (boxes), and maximum and minimum (whiskers) from three independent experiments. P-values shown were obtained using the Mann–Whitney test. Scale bars: 50 µm.