Fig. 3.

Interference with β1 and αV integrins abolishes migration of glioma cells on rBM but not astrocyte-deposited matrix. (A) Overviews and (B) average migration distance of U-251 and E-98 cells from spheroids after 24 hours on mouse astrocyte-deposited matrix (mADM) or rBM in the presence of isotypic IgG and cRADfV control peptide or the adhesion-perturbing antibodies 4B4 (β1), 17E6 (αV) and GoH3 (α6), and cRGDfV peptide. Mouse astrocytes were cultured for 3 days and (B) removed from culture surface with 0.002 M EDTA only, or (C) the culture surface was additionally treated with 0.25% Triton X-100 and 0.25% sodium deoxycholate in PBS. (D) Overviews and (E) average migration distance of E-98 cells from spheroids on mouse (mADM) or human (hADM) astrocyte-deposited matrix. Mouse and human astrocytes were cultured for 3 or 7 days, respectively, and removed with 0.002 M EDTA followed by treatment of culture surface with 0.25% Triton X-100 and 0.25% sodium deoxycholate in PBS. Data represent on average 10-22 spheroids per condition from three independent experiments. Values display the median (black line), 25th and 75th percentiles (boxes), and maximum and minimum (whiskers). P-values shown were obtained using the Mann-Whitney test. n.s., not significant. Scale bars: 200 µm.