Fig. 3.

PI4KIIIα is required for normal levels of PI4P and PI(4,5)P₂ at the plasma membrane. (A) Representative images of the fluorescent deep pseudopupil from 1-day-old flies expressing the P4M::GFP probe with genotype as indicated. (B) Representative images of the fluorescent deep pseudopupil from flies expressing the PLCδPH::GFP probe with genotype as indicated. The experiments in A and B were repeated two times, and one of the trials is shown here. (C,D) Quantification of fluorescence intensity of the deep pseudopupil. Fluorescence intensity per unit area is shown on the y-axis (A.U., arbitrary units); the x-axis indicates the genotype. Values are mean±s.e.m. n=10 flies per genotype. *P<0.05 (two-tailed unpaired t-test). (E,F) Western blot of head extracts made from flies expressing the P4M::GFP and PLCδPH::GFP probes, respectively. The levels of tubulin are used as a loading control; genotypes are indicated above.