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(A) In-situ gel-based ABPP fluorescent scan of Mtb Pks13TE wt and S1533A mutant in E. coli with EZ120P. (B, C) Intact-protein MS of purified PKS13 wt and S1533A of Mtb with EZ120. (D) Immunoblot analysis of Ag85A and (E) loading control (background protein band) in wt, knock-out and complement Msmeg strain. (F) In situ gel-based ABPP fluorescent scan of Msmeg wt and ΔAg85A insoluble protein fractions with EZ120P. IPTG: isopropyl β-D-1-thiogalactopyranoside; MW: molecular weight.
