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. 2018 Aug 22;13(8):e0201122. doi: 10.1371/journal.pone.0201122

Fig 3. Environmentally relevant doses of BPA applied throughout differentiation inhibit adipogenesis.

Fig 3

Confluent 3T3-L1 pre-adipocytes were exposed to different concentrations of BPA (0.1-3nM) or vehicle (0.1% ethanol) from day -2 to day 7. Suboptimal adipocyte differentiation was induced at day 0 with media enriched with dexamethasone and insulin (DI). (A) Schematic representation of the treatment dose and time course applied. (B) Triglyceride accumulation visualized by oil Red O staining and representative bright field microscopy images (40X magnification) were acquired between day 8–9. (C) Quantitative reverse transcription PCR (qRT-PCR) analysis of adipocyte marker gene expression was performed between day 8–9. Gene expression was normalized to 36B4 and is presented as relative mRNA expression level. *p≤ 0.05,**p≤0.01 versus vehicle treated cells exposed to same protocol.