Table 3. Primer sequences of selected DEGs used for real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR).
Genes | Primer names | Forward (5’-3’) | Reverse (5’-3’) | Product size |
---|---|---|---|---|
1. Ubiquitin 10 | UBQ10 | tgcatctcgttctccgattg | gcgaagatcagtcgttgttgc | 166 bp |
2. GTP binding | GTPb | gttgccttcttttgcgtttct | gcaatttgatccgttttccat | 114 bp |
3. 2-Oxyglutarate (2OG) and FE(II)-dependent oxygenase superfamily protein | 2OG-FE(II) | ggagttgaggttgctgaaga | ggaaggaggagaagaagaaga | 146 bp |
4. HSP20-like chaperones superfamily protein | HSP20 | aaaaacccacaagcccaa | ctctcactttctcttcatcttc | 190 bp |
5. Myb domain protein 4 | MYB | ggtgagggtggtttaagt | ctctagatttgcgtttgatg | 161 bp |
6. Glycosyl hydrolases family 32 | GH32 | gattaggcgttgaagaag | gaacaggagctgaagcta | 145 bp |
7. Cytochrome P450, family 94, subfamily B, polypeptide 3 | CYP450 | actcccacttatcctacac | caacgaaaccacaaccaa | 120 bp |
8. Alpha/beta-hydrolases protein family | Alpha/B | cactctctcttctacttcttct | ttttcccttgtggccctt | 179 bp |
9. GDSL-like Lipase/Acylhydrolase family protein | GDSL | ggttttgtaatggagtgg | tattttgggtgtgagagg | 176 bp |
10. Terpene synthase 21 | Terp | tcttcttcttctcctttcctc | tccccacttacaatcccc | 141 bp |
11. Gibberrelin 2-oxidase 6 | Gibb | tgcatgattcctgactagtc | catggatcagtctagaatg | 150bp |