. 2018 Aug 22;13(8):e0202324. doi: 10.1371/journal.pone.0202324

Table 1. Effects of two basal media MS and DKW augmented with different combinations and concentrations of plant growth regulators (PGRs) on the induction of embryogenic callus from two different explants (root and leaf) of H. sabdariffa L. var. HS 4288.

Basal culture medium	Concentration of PGRs (μM)				Percentage of explants forming embryogenic callus
	2,4-D	KIN	NAA	BA	Root	Leaf
MS	0.0	0.0	0.0	0.00	0.00^m	0.00^m
		2.32	-	-	46.67(43.28)^cdef	26.67(31.31)^hijk
	2.26	4.65	-	-	50.00(45.00) ^cdef	30.00(33.21)^ghij
		9.30	-	-	53.33(46.72)^cde	36.67(37.46)^fghi
		-	-	2.22	23.33(28.66)^ijk	6.67(15.34)^lm
	2.26	-	-	4.44	36.67(37.46)^fghi	20.00(26.57)^jkl
		-	-	8.88	40.00(39.23)^efgh	26.67(31.31)^hijk
	-	-		2.22	6.67(15.34)^lm	0.00^m
	-	-	2.68	4.44	16.67(24.35)^jkl	0.00^m
	-	-		8.88	20.00(26.57)^jkl	6.67(15.34)^lm
DKW	0.0	0.0	0.0	0.00	0.00^m	0.00^m
		2.32	-	-	60.00(50.77)^abc	43.33(40.98)^defg
	2.26	4.65	-	-	70.00(56.79)^a	53.33(46.72)^cde
		9.30	-	-	66.67(54.94)^ab	56.67(49.02)^bcd
		-	-	2.22	36.67(37.46)^fghi	16.67(24.35)^jkl
	2.26	-	-	4.44	46.67(43.28)^cdef	26.67(31.31)^hijk
		-	-	8.88	46.67(43.28)^cdef	30.00(33.21)^ghij
	-	-		2.22	16.67(24.35)^jkl	6.67(15.34)^lm
	-	-	2.68	4.44	30.00(33.21)^ghij	13.33(21.13)^klm
	-	-		8.88	26.67(31.31)^hijk	16.67(24.35)^jkl

Values are expressed as means of three replicated experiments, each with ten explants per treatment. Means followed by the same letters in superscript within columns are not significantly different at p ≤0.05 level according to Duncan multiple range test (DMRT). Values in the parentheses are the arc sine transformations of the percentage data. All data were taken after 4 weeks of culture.