Table 2. Influence of various concentrations of plant growth regulators (PGRs) supplemented media on somatic embryos development from root derived embryogenic callus of H. sabdariffa var. HS 4288.
| Basal culture medium | Concentration of PGRs (μM) | Average number of somatic embryos | ||||
|---|---|---|---|---|---|---|
| 2,4-D | KIN | NAA | BA | Globular | Cotyledonary | |
| 0.0 | 0.0 | 0.0 | 0.00 | 0.00n | 0.00i | |
| 2.32 | - | - | 6.83±0.15f | 2.10±0.12fg | ||
| 2.26 | 4.65 | - | - | 7.53±0.18e | 2.37±0.32ef | |
| 9.30 | - | - | 8.80±0.17d | 2.53±0.20e | ||
| MS | - | - | 2.22 | 2.73±0.12l | 0.00i | |
| 2.26 | - | - | 4.44 | 3.23±0.15k | 0.00i | |
| - | - | 8.88 | 4.03±0.18ij | 1.37±0.12h | ||
| - | - | 2.22 | 2.27±0.12m | 0.00i | ||
| - | - | 2.68 | 4.44 | 3.20±0.21k | 0.00i | |
| - | - | 8.88 | 4.03±0.17ij | 0.00i | ||
| 0.0 | 0.0 | 0.0 | 0.00 | 0.00n | 0.00i | |
| 2.32 | - | - | 11.13±0.12c | 6.87±0.20c | ||
| 2.26 | 4.65 | - | - | 14.53±0.23a | 8.90±0.17a | |
| 9.30 | - | - | 13.03±0.19b | 7.68±0.12b | ||
| DKW | - | - | 2.22 | 3.17±0.2k | 0.00i | |
| 2.26 | - | - | 4.44 | 4.07±0.12h | 1.87±0.15g | |
| - | - | 8.88 | 6.10±0.17g | 3.20±0.21d | ||
| - | - | 2.22 | 2.70±0.15lm | 0.00i | ||
| - | - | 2.68 | 4.44 | 3.73±0.12j | 0.00i | |
| - | - | 8.88 | 4.10±0.12hi | 1.97±0.12g | ||
Values are expressed as mean ± standard error (±SE) of three replicated experiments, each with ten explants per treatment. Means followed by the same letters in superscript within columns are not significantly different at p ≤ 0.05 level according to Duncan multiple range test (DMRT). All data were taken after 6 weeks of culture.