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Flow karyotype of the Ishikawa 3-H-12 cell line at P25* without removing the background manually by image processing was obtained after sorting a larger number of chromosomes than usual to show clearly the der(9) peak close to the normal chromosome 3 peak. The der(9) peak is weaker than other peaks that have pairs of normal chromosomes as it exists in only one copy in each cell. Normal chromosome 9 and one of the chromosome 14 homologues, 14S, are also selected for sorting.
