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Detection of mono-allelic genome editing by ORNi-PCR. (A) ORNi-PCR with gDNAs extracted from WT and genome-edited Raji cells. To mimic mono-allelic mutations, gDNAs of WT and T4 or T9 were mixed at 1:1 ratio. PCR was performed in the presence (1 µM) or absence of ORNs. M: molecular weight marker. (B) DNA-sequencing signals of PCR products. PCR products of WT + T4 in (A) [(−) ORN and ORN_20b] were purified from agarose gels and subjected to DNA sequencing.
