Figure 6.

Screening of genome-edited cells by ORNi-PCR. (A) Schematic of screening of genome-edited cells by ORNi-PCR. (B) Target position of genome editing and sequence of a CDKN2A(p16)-specific ORN. The forward and reverse DNA sequences of the allele are shown. The CRISPR target site in 293T is underlined, and PAM positions are shown in red. A black arrow indicates the cleavage sites of CRISPR. (C) Setting of ORNi-PCR with gDNA extracted from 293T. M: molecular weight marker. (D) Results of ORNi-PCR (1 µM ORN). (E) Schematic of confirmation of indel mutations. (F) DNA sequences around the CRISPR target site in the CDKN2A(p16) locus in genome-edited cells. The forward DNA sequences of both alleles are shown.