Table 1.

Comparison of genome-editing detection methods

Methods	Time (h)	Accuracy	Labor	Cost	Limitation
Mismatch cleavage assaya	>4	Moderate	Moderate	Moderate	Not suitable for detection of mutations that do not form DNA bulges at mismatches in the assay (e.g. homozygous bi-allelic mutations).
PCR-based methoda	1–2	High	Low	Low	—
ORNi-PCR	1–2	High	Low	Low	It may be difficult to distinguish mono- and bi-allelic mutations by endpoint ORNi-PCR.

^aProperties of these methods have been previously described.¹³