Table 2.
Details on 10 SSR markers used for amplification of teak germplasm
| SSR code | SSR Motif | Primers (5’-3’) | Annealing temperature (oC) | Number of alleles | Product size (bp) |
|---|---|---|---|---|---|
| IFT83 | (AG)21 | F5’AATTGGCATAAAGCGTGCTACTR5’CGCACGTCCTATTTTGGTTTAT | 54 | 5 | 312-393 |
| IFT821 | (AC)24 | F5’CCCCAATTATGTCAACCGACT R5’GGCATTATCTAAGATCGCAAGG | 53.9 | 3 | 331-350 |
| IFT63 | (ATG)12 | F5’CCCAAAGCGAATAATATCCTAC R5’CATGACTTGTTCGATGGGCTAAT | 54 | 3 | 250-275 |
| IFT168 | (TCT)12 | F5’ATCTTCAGCAGAGGAGGCTATG R5’GTGCCCTTTTCTCTCTTCTTCA | 55 | 4 | 287-306 |
| IFT479b | (GGA)11 | F5’GTGAAGATTCGGGTATGGAGAG R5’TACTCCCAGATTTCCCAATCAC | 55 | 3 | 331-343 |
| IFT382 | (TATG)7 | F5’TACTCATCACTGTCCCCAGTTG R5’GAACGGGAATCTAGAGTTGTGG | 56 | 3 | 337-350 |
| IFT 28 | (AAAG)6 | F5’CAGCCTCTGCATGTCAAATAAA R5’TTAGAGCTGGATATGCCATTGA | 53.6 | 3 | 381-393 |
| IFT14 | (TTCT)9 | F5’TGTGGTATTGGACCATCTGAAA R5’GGTAACCCACCAACAAATATGC | 54 | 5 | 265-278 |
| IFT3 | (GAAAG)5 | F5’TTCCACCTACTGGTTAAGGAAC R5’ATGGCTTACCAATTTACCAAACC | 54 | 1 | 330 |
| IFT777 | (TCAGG)6 | F5’TACTAACCGGAAGAGGGAAACC R5’TGTCGCTATGGACAGTTCATCT | 56 | 4 | 312-343 |