Figure 1.

Illustration of methods. (a) Iso-Seq workflow for data processing (ToFU). (b) Mapping of PacBio data. (c) Identification of full-length. Same 5′ and 3′ ends: the first and last splice sites of FLNC were as same as the reference annotation transcripts. With 5′ and 3′ ends: the first and last splice sites of the reference transcripts were presented in the splice sites of the FLNC sequence. Same 5′ end: The first splice sites of 5′ end was identical between annotated transcript and FLNC sequence. With 5′ end: the first splice site of the 5′ end of the annotated transcript was presented in the FLNC sequence. The criteria of full-length were the type ‘with 5′ end’. (d) Criteria for assessing a new isoform. (e) Fusion transcript identification from PacBio sequences.