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. 2018 May 29;25(4):421–437. doi: 10.1093/dnares/dsy014

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© The Author(s) 2018. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Validation of differential expressed isoforms. (a) Heatmap of differential expressed isoforms in eight tissues. (b) Validation of differential expressed isoforms by RT-PCR. The isoform symbol was explained, for example, 8.1373.1 represented the first transcript of loci 8.1373 on the chromosome 8. 8.1373.1, 14.1634.2 and 15.158.2 belonged to the transcripts of novel loci; 5.203.1, 4.1478.1 and 1.1932.4 were known isoforms of annotated genes of SSC10.2 reference; 13.681.3, 1.3863.6 and 11.632.1 were new isoforms came from the annotated genes of SSC10.2 reference; 1.1177.1 and x.631.1 were novel isoforms of novel lncRNAs; 1.2355.7 and 7.168.3 were fusion transcripts and the genes below them represented their precursor genes.