Fig. 2.

Effects of ABL and TPTD on bone resorption-related factors in osteoblastic cells. a Schema of the experiment. SaOS-2 was incubated with either ABL or TPTD at a concentration of 100 nmol/L on day 10. Six hours after treatment, the medium was replaced with peptide-free or peptide-containing medium for the rest of the experimental period. b, c Analysis of relative gene expression and protein secretion by qRT-PCR and ELISA under continuous treatment. d, e Analysis of relative gene expression and protein secretion by qRT-PCR and ELISA under transient treatment. *P < 0.05, **P < 0.01, ***P < 0.001. N.S. not significant. n = 3 replicate wells per group. Data are representative of two independent experiments