Table 1. Proteins with significantly changed abundance in skeletal muscle and adipose tissue in response to acute sleep loss in humans.
Proteins (A) up-regulated and (B) down-regulated in skeletal muscle (vastus lateralis muscle) and up-regulated in (C) subcutaneous adipose tissue in response to sleep loss compared with normal sleep. No proteins were found to be down-regulated in subcutaneous adipose tissue. Protein IDs shown are limited to 3. n = 15 pairs for each tissue; two-sided t tests.
| Protein IDs | Protein names (gene name in parentheses) |
Log2 ratio (sleep loss/sleep) |
SEM | P |
| A. Up-regulated proteins in skeletal muscle | ||||
| O14558 | Heat shock protein beta-6 (HSPB6) | 0.33 | 0.43 | 0.012 |
| P07195 | l-Lactate dehydrogenase B chain; l-lactate dehydrogenase (LDHB) | 0.37 | 0.51 | 0.017 |
| P08238 | Heat shock protein HSP 90-beta (HSP90AB1) | 0.12 | 0.16 | 0.018 |
| P35609; P35609-2 | Alpha-actinin-2 (ACTN2) | 0.09 | 0.13 | 0.033 |
| Q2TBA0; Q2TBA0-2 | Kelch-like protein 40 (KLHL40) | 0.38 | 0.55 | 0.035 |
| Q9GZV1 | Ankyrin repeat domain-containing protein 2 (ANKRD2) | 0.44 | 0.72 | 0.038 |
| Q5XKP0 | Protein QIL1 (QIL1) | 0.22 | 0.26 | 0.040 |
| C9JFR7; P99999 | Cytochrome c (CYCS) | 0.21 | 0.32 | 0.042 |
| O14949 | Cytochrome b-c1 complex subunit 8 (UQCRQ) | 0.23 | 0.37 | 0.049 |
| B. Down-regulated proteins in skeletal muscle | ||||
| O00757 | Fructose-1.6-bisphosphatase isozyme 2 (FBP2) | −0.27 | 0.28 | 0.003 |
| Q9NR12-6 | PDZ and LIM domain protein 7 (PDLIM7) | −0.31 | 0.35 | 0.005 |
| P00558; P00558-2 | Phosphoglycerate kinase 1 (PGK1) | −0.22 | 0.22 | 0.006 |
| Q9UKS6 | Protein kinase C and casein kinase substrate in neurons protein 3 (PACSIN3) | −0.18 | 0.19 | 0.011 |
| P02585 | Troponin C, skeletal muscle (TNNC2) | −0.27 | 0.32 | 0.012 |
| P14543-2; P14543 | Nidogen-1 (NID1) | −0.31 | 0.35 | 0.013 |
| P14618-2 | Pyruvate kinase (PKM) | −0.21 | 0.26 | 0.015 |
| Q08043 | Alpha-actinin-3 (ACTN3) | −0.40 | 0.47 | 0.017 |
| Q5T7C4; Q5T7C6; P09429 | High mobility group protein B1 (HMGB1) | −0.19 | 0.23 | 0.019 |
| Q8N142; Q8N142-2 | Adenylosuccinate synthetase isozyme 1 (ADSSL1) | −0.14 | 0.20 | 0.022 |
| P61586; Q5JR08; P08134 | Transforming protein RhoA; Rho-related guanosine 5′-triphosphate–binding protein RhoC (RHOA; RHOC) |
−0.17 | 0.22 | 0.024 |
| P55786; P55786-2 | Puromycin-sensitive aminopeptidase (NPEPPS) | −0.08 | 0.11 | 0.026 |
| Q14324 | Myosin-binding protein C, fast-type (MYBPC2) | −0.37 | 0.56 | 0.026 |
| P04075 | Fructose-bisphosphate aldolase A; Fructose-bisphosphate aldolase (ALDOA) | −0.14 | 0.20 | 0.027 |
| P50995-2; P50995 | Annexin A11 (ANXA11) | −0.33 | 0.28 | 0.028 |
| P62942; Q5W0X3 | Peptidyl-prolyl cis-trans isomerase FKBP1A; peptidyl-prolyl cis-trans isomerase (FKBP1A; FKBP12-Exip2) |
−0.12 | 0.17 | 0.030 |
| P17612; P17612-2; P22694-4 | cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit beta (PRKACA; PRKACB; KIN27) |
−0.13 | 0.19 | 0.032 |
| P00338; P00338-3; P00338-4 | l-Lactate dehydrogenase A chain (LDHA) | −0.21 | 0.31 | 0.036 |
| P07108; P07108-3; P07108-2 | Acyl-CoA–binding protein (DBI) | −0.17 | 0.26 | 0.037 |
| P12882 | Myosin-1 (MYH1) | −0.93 | 1.38 | 0.038 |
| P08237; P08237-3; P08237-2 | 6-Phosphofructokinase, muscle type (PFKM) | −0.16 | 0.26 | 0.040 |
| P05976 | Myosin light chain 1/3, skeletal muscle isoform (MYL1) | −0.16 | 0.24 | 0.041 |
| Q0VAK6; Q0VAK6-2 | Leiomodin-3 (LMOD3) | −0.23 | 0.31 | 0.043 |
| C. Up-regulated proteins in subcutaneous adipose tissue | ||||
| P09211 | Glutathione S-transferase (GSTP) | 0.23 | 0.06 | 0.004 |
| P02788 | Lactotransferrin (LTF) | 0.95 | 0.26 | 0.004 |
| P00558; P00558-2 | Phosphoglycerate kinase 1 (PGK1) | 0.23 | 0.08 | 0.011 |