Table 3.
Summary of exclusion criteria for Langendorff perfused hearts
| Parameter | Mouse heart | Rat heart (250–400 g bw) |
|---|---|---|
| Baseline (prior to any intervention, e.g. IPC) | ||
| Time to perfusion | > 3 min | > 3 min |
| Coronary flow | < 2 or > 5.5 ml/min | < 10 or > 28 ml/min |
| Arrhythmias | > 10 ectopics during 10 min baseline recordings (VT or VF should not occur) | > 10 ectopics during 10 min baseline recordings (VT or VF should not occur) |
| Heart rate | < 300 bpm | < 200 or > 400 bpm |
| Left ventricular end-diastolic pressure | < 5 or > 10 mmHg | < 5 or > 10 mmHg |
| Left ventricular developed pressure | < 60 or > 140 mmHg | < 70 or > 140 mmHg |
| Temperature | 37 ± 0.5 °C (< 36 °C or > 38 °C for > 1 min) | 37 ± 0.5 °C (< 36 °C or > 38 °C for > 1 min) |
| Reperfusion | ||
| Coronary flow | ≤ ischemic flow | ≤ ischemic flow |
| Arrhythmia duration (ventricular tachycardia or fibrillation) | > 2 min (intervene immediately—flicking, cold buffer or KCl) | > 2 min (intervene immediately—flicking, cold buffer or KCl) |
| Heart rate | < 150 bpm (irrelevant if pacing) | |
| Infarct criteria | ||
| Area at risk | n/a for global ischemia | n/a for global ischemia; for regional ischemia: < 40 or > 70% of ventricular tissue |