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. 2018 Jun 20;8(12):e2883. doi: 10.21769/BioProtoc.2883

Figure 6. Image adjustment and counting of skeletal muscle fiber number within a cross-section.

Figure 6.

A. Original image, prior to manipulation of the display settings; B. The same image following adjustment of the display to increase the visible background staining, allowing for the identification and counting of skeletal muscle fibers; C. Count of individual muscle fibers, denoted by yellow Xs; D. Fibers were manually outlined to help demarcate individual skeletal muscle fiber borders (white) along with the fiber count from panel C (yellow Xs). A-D) CD11b (green), CD206 (red), cell nuclei/DAPI (blue). Scale bars = 100 µm.