Figure 10. Skeletal muscle stem cells (satellite cells) can weakly express CD206 but are easily distinguished from macrophages.

A. Representative image from human vastus lateralis showing a Pax7+ satellite cell (green, B), a CD206+ macrophage (red, C) and a Pax7+/CD206^weak satellite cell (D). Cell nuclei are stained with DAPI (blue). Scale bars = 100 µm. B-D. Higher magnification of the boxes from panel A. Note the overexposure of CD206 macrophage staining (C) when the intensity is adjusted so that CD206 expression in satellite cells is visible (D). Images acquired as Z stacks with a 40x objective. Scale bars = 20 µm. E. High magnification images from human vastus lateralis muscle showing a CD206+ muscle macrophage (red) in close proximity to a Pax7+ satellite cell (green). Cell nuclei are stained with DAPI and skeletal muscle fiber borders are stained with Laminin (both in blue). Images were acquired as Z stacks with a 100x oil objective. Scale bars = 20 µm.