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. 2018 Jun 20;8(12):e2883. doi: 10.21769/BioProtoc.2883

Table 1. Overview of monocyte and macrophage markers.

Marker Synonyms Tissue cell populations Monocyte/macrophage subset Notes
CD11b CR3, C3biR, Integrin alphaM, ITGAM, Mac-1, Mo1 B cells, dendritic cells, granulocytes, macrophages, monocytes, NK cells and T cells Pan-macrophage
*CD14 Monocyte differentiation antigen CD14, LPS receptor B cells, dendritic cells, granulocytes, macrophages and monocytes Pan-monocyte *Co-expression with CD16 identifies monocyte subsets
*CD16 FcγRIIIA B cells, macrophages, monocytes, neutrophils, NK cells T cells

CD14++/CD16- - classical

CD14++/CD16+ - intermediate

CD14+/CD16++ - non-classical

CD68 gp110, LAMP4, Macrosialin, SCARD1 B cells, dendritic cells, endothelial cells, fibroblasts, granulocytes, hematopoietic stem cells (LT), macrophages, monocytes, NK cells and T cells Pan-macrophage Intracellular expression
CD80 B7, B7-1, B7.1 BB1, CD28LG, CD28LG1, LAB7 B cells, dendritic cells, macrophages, monocytes and T cells M1 Co-stimulatory with CD86
CD86 B7-2, B7.2, B70, CD28LG2, LAB72 B cells, dendritic cells, macrophages, monocytes and T cells M1
CD163 D11, D1 1C163A, GHI/61, M130, MM130, RM3/1, SCARI1 B cells, macrophages and monocytes M2c Identifies a subset of CD206+ macrophages in skeletal muscle
CD206 CLEC13D, mannose receptor, MMR, MRC1 Dendritic cells, certain skin cells, endothelial cells, macrophages and monocytes M2 In rare instances, Pax7+ skeletal muscle satellite cells co-express CD206
Markers in non-injured human skeletal muscle
CD11b+/CD206- M1-like macrophages FC - Also expresses CD14
CD11b+/CD206+ M2-like macrophages FC - Also express CD14 and CD86
CD163+/CD206+ M2c macrophages
FC = multichannel flow cytometry analysis