Table 1. Overview of monocyte and macrophage markers.
Marker | Synonyms | Tissue cell populations | Monocyte/macrophage subset | Notes |
---|---|---|---|---|
CD11b | CR3, C3biR, Integrin alphaM, ITGAM, Mac-1, Mo1 | B cells, dendritic cells, granulocytes, macrophages, monocytes, NK cells and T cells | Pan-macrophage | |
*CD14 | Monocyte differentiation antigen CD14, LPS receptor | B cells, dendritic cells, granulocytes, macrophages and monocytes | Pan-monocyte | *Co-expression with CD16 identifies monocyte subsets |
*CD16 | FcγRIIIA | B cells, macrophages, monocytes, neutrophils, NK cells T cells |
CD14++/CD16- - classical CD14++/CD16+ - intermediate CD14+/CD16++ - non-classical |
|
CD68 | gp110, LAMP4, Macrosialin, SCARD1 | B cells, dendritic cells, endothelial cells, fibroblasts, granulocytes, hematopoietic stem cells (LT), macrophages, monocytes, NK cells and T cells | Pan-macrophage | Intracellular expression |
CD80 | B7, B7-1, B7.1 BB1, CD28LG, CD28LG1, LAB7 | B cells, dendritic cells, macrophages, monocytes and T cells | M1 | Co-stimulatory with CD86 |
CD86 | B7-2, B7.2, B70, CD28LG2, LAB72 | B cells, dendritic cells, macrophages, monocytes and T cells | M1 | |
CD163 | D11, D1 1C163A, GHI/61, M130, MM130, RM3/1, SCARI1 | B cells, macrophages and monocytes | M2c | Identifies a subset of CD206+ macrophages in skeletal muscle |
CD206 | CLEC13D, mannose receptor, MMR, MRC1 | Dendritic cells, certain skin cells, endothelial cells, macrophages and monocytes | M2 | In rare instances, Pax7+ skeletal muscle satellite cells co-express CD206 |
Markers in non-injured human skeletal muscle | ||||
CD11b+/CD206- | M1-like macrophages | FC - Also expresses CD14 | ||
CD11b+/CD206+ | M2-like macrophages | FC - Also express CD14 and CD86 | ||
CD163+/CD206+ | M2c macrophages | |||
FC = multichannel flow cytometry analysis |