Table 2. Detailed IHC Antibody Information.
| Primary Antibody | Company | Catalog Number | Reactivity | Host /Isotype | Dilution/Diluent |
|---|---|---|---|---|---|
| CD68 | Dako | M0814 | Human/Mouse/Rat/Monkey | Mouse/IgG1 | (1:100)/2.5% NHS |
| CD11b | Cell Sciences | MON1019 | Human | Mouse/IgG1 | (1:100)/2.5% NHS |
| Purified IgG1, κ | BD | 555746 | N/A | Mouse/IgG1 | (1:500)/2.5% NHS |
| C206 | R&D Systems | AF2534 | Human | Goat/IgG (polyclonal) | (1:200)/2.5% NHS |
| CD163 | Hycult Biotech | HM2157 | Human/Monkey | Mouse/IgG1 | (1:50)/2.5% NHS |
| Pax7 | Developmental Studies Hybridoma Bank | Pax7 | Human/Mouse/Rat | Mouse/IgG1 | (1:100)/2.5% NHS |
| Biotinylated goat anti-mouse IgG1 | Jackson ImmunoResearch | 115-065-205 | Mouse IgG1 | Goat/N/A | (1:1,000)/2.5% NHS |
| ImmPRESS –AP | Vector Laboratories | MP-5402 | Mouse IgG | N/A | Neat/no dilution |
| Biotinylated rabbit anti-goat IgG | Vector Laboratories | BA-5000 | Goat | Rabbit/N/A | (1:500)/2.5% NHS |
| Streptavidin HRP (SA-HRP) | Thermo Fisher Scientific | S911 | Biotin | N/A | (1:500)/PBS |
| Streptavidin Alexa Fluor 594 (SA-594) | Thermo Fisher Scientific | S32356 | Biotin | N/A | (1:200)/PBS |
| Superboost TSA Alexa Fluor 488 (TSA 488) | Thermo Fisher Scientific | B40953 | HRP | N/A | (1:500)/PBS |
| ImmPACT Vector Red kit | Vector Laboratories | SK-5105 | Alkaline Phosphatase | N/A | According to manufacturer’s instructions |