Table 3. Detailed antibody information for multichannel flow cytometry.
| Antigen | Fluorophore | Laser | Filter/Bandpass | Ex (nm) | Em (nm) | Concentration (µg/µl) | µl/106 cells (500 µl total volume) | Company | Catalog Number |
| CD11b | Alex Fluor 488 | Blue (488) | 530/30 | 490 | 525 | 0.4* | 25 | BioLegend | 301317 |
| CD14 | Pacific Blue | Violet (407) | 450/50 | 410 | 455 | 0.5 | 20 | BioLegend | 325615 |
| CD16 | VioGreen | Violet (407) | 525/50 | 405 | 520 | NA | 50 | Miltenyi Biotec | 130-113-959 |
| CD86 | Phycoerythrin (PE) | Blue (488) | 575/26 | 496 | 578 | 0.1* | 25 | BioLegend | 305405 |
| CD206 | PerCP/Cy5.5 | Blue (488) | 695/40 | 482 | 695 | 0.1* | 25 | BioLegend | 321121 |
| LIVE/DEAD | Fixable Blue | UV (325) | 450/50 | 350 | 450 | NA | 0.5 | Thermo Fisher Scientific | L34961 |
| Mouse IgG1, κ | Alexa Fluor 488 | Blue (488) | 530/30 | 490 | 525 | 0.2* | 50 | BioLegend | 400132 |
| Mouse IgG1, κ | Pacific blue | Violet (407) | 450/50 | 410 | 455 | 0.5 | 20 | BioLegend | 400131 |
| REA Control (S) | VioGreen | Violet (407) | 525/50 | 405 | 520 | NA | 50 | Miltenyi Biotec | 130-104-608 |
| Mouse IgG2b, κ | Phycoerythrin (PE) | Blue (488) | 575/26 | 496 | 578 | 0.2 | 12.5 | BioLegend | 400311 |
| Mouse IgG1, κ | PerCP/Cy5.5 | Blue (488) | 695/40 | 482 | 695 | 0.2* | 12.5 | BioLegend | 400149 |
| *Concentration is not specific and varies by batch | |||||||||