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Fluorescence intensity of DiR/np with different DiR loading suspended in mouse serum at different NP concentrations, observed at different time points. (a) DiR3.6/np and (b) DiR0.5/np. The fluorescence intensity was quantified by the SPECTRAL AMI Imaging System. Error bars: s.d. of 3 readings of the same sample. The measurement was repeated with two independently prepared batches of NPs at selected concentrations and presented in Fig. S21 and Fig. S22.
