Skip to main content
. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: FEBS J. 2018 Jun 14;285(14):2691–2707. doi: 10.1111/febs.14514

Fig. 2.

Fig. 2.

Both the PYD and CARD of ASC are required for speck formation. (A) Left side: subcellular localization of the AcGFP-fused hASC, zASC, zASCPYD, zASCCARD and AcGFP-N1 proteins. Bars: 10 μm. Right side: ASC SPECK formation close to nucleus per cell. 4’,6-Diamidino-2-phenylindole (DAPI) staining was used to label nucleus. (B) Representative confocal images of the zebrafish embryo yolk circulation region when zASC and zASCPYD are overexpressed. Bars: 50 and 100 μm. (C) Representative electron micrograph of the zASC, zASCPYD and zASCCARD proteins subjected to negative staining. Bar, 100 nm.