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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Stem Cells. 2018 Apr 22;36(8):1198–1209. doi: 10.1002/stem.2833

Figure 5. The NFκB subunit RelA binds to the promoters of cardiomyocyte maturation genes.

Figure 5

(A) Neonatal cardiac fibroblasts were transfected with negmiR or miR combo. After 7 days, chromatin DNA was subjected to ChIP analysis. Primers were designed to target the first 1Kb of the indicated cardiomyocyte sarcomere genes (represented by -01Kb). Results are presented as the fold enrichment in RelA binding where percent input of the negmiR control was taken to be 1. N=3 independent experiments. Data represented as Mean ± SEM. Comparisons are made between miR combo and negative control miR (negmiR), **P<0.01, *P<0.05, ns: not significant.

(B) Neonatal cardiac fibroblasts were transfected with miR combo and either a control siRNA or a siRNA that targeted RelA. After 7 days, chromatin DNA was subjected to ChIP analysis. Primers were designed to target the first 1Kb of the indicated cardiomyocyte sarcomere genes (represented by -01Kb). Results are presented as the percentage of chromatin input. N=3 independent experiments. Data represented as Mean ± SEM.